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Bolton visited Prenton Park last season for a pre-season game and won a poor match 1-0, although rivalries between to the two sides run back into the 1990's as both teams met each other in a number of crucial league and cup matches.
As per usual, Rovers will also be playing the usual local non-league opposition, with the first team set to make trips to Heswall, Marine, Cammell Laird, Vauxhall Motors, Southport and League Two side Morecambe.
For the full pre-season fixture list, please click here